microsomal triglyceride transfer protein mttp  (Affinity Biosciences)

Journal: Bioengineered

Article Title: Caffeic acid dimethyl ether alleviates alcohol-induced hepatic steatosis via microRNA-378b-mediated CaMKK2-AMPK pathway

doi: 10.1080/21655979.2022.2060586

Figure Lengend Snippet: CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice. (a,b) Western blot analysis of the protein expressions of SREBP-1c, FASN, PPARα, CPT1, MTTP and protein ratio of p-foxO1/foxO1 when the mice were injected with AAV-miR-378b agomir and antagomir. All data are expressed as the means ± SD of at least three separate experiments. #p < 0.05, ##p < 0.01 vs. NC group of AAV-miR-378b agomir or antagomir. *p < 0.05, **p < 0.01 vs. AAV-miR-378b agomir or antagomir.

Article Snippet: Antibodies against β-actin, p-CaMKK2, CaMKK2, Peroxisome Proliferator Activated Receptor α (PPARα), Microsomal Triglyceride Transfer Protein (MTTP), FASN, Sterol Regulatory Element Binding Protein 1c (SREBP-1c), Carnitine Palmitoyl Transferase 1 (CPT1), Forkhead box other 1 (foxO1), and p-foxO1 were purchased from Affinity Biosciences (OH, USA).

Techniques: Western Blot, Injection

Journal: Industrial Health

Article Title: Inhalation exposure to 2-ethyl-1-hexanol causes hepatomegaly and transient lipid accumulation without induction of peroxisome proliferator-activated receptor alpha in mice

doi: 10.2486/indhealth.2020-0252

Figure Lengend Snippet: Relative protein expression levels in the liver of mice exposed to 2EH for 3 months (3 M) or 6 months (6 M). (A) Representative bands of MTTP and GAPDH after western blot analysis in mice exposed to 2EH for 3 M. GAPDH was used as an internal staining standard. For each band, (B) catalase, (C) BP, (D) VLCAD, and (E) MTTP were quantified by densitometric analysis. The asterisk indicates statistical significance (* p <0.05) when compared with the respective control. Data are presented as mean ± SD (n=6 for 100 ppm and n=7 each for the other groups). 2EH, 2-Ethyl-1-hexanol; MTTP, microsomal triglyceride transfer protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VLCAD, very long-chain acyl-CoA dehydrogenase; BP, peroxisomal bifunctional protein.

Article Snippet: The primary antibodies used for western blot analyses were as follows: microsomal triglyceride transfer protein (MTTP) (Aviva System Biology Corporation, San Diego, California, USA), catalase (Abcam plc., Cambridge, Cambridgeshire, UK), peroxisomal bifunctional protein (BP) ) , very-long-chain acyl-CoA dehydrogenase (VLCAD) ) , and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Inc., Danvers, Massachusetts, USA).

Techniques: Expressing, Western Blot, Staining, Control

Journal: Industrial Health

Article Title: Inhalation exposure to 2-ethyl-1-hexanol causes hepatomegaly and transient lipid accumulation without induction of peroxisome proliferator-activated receptor alpha in mice

doi: 10.2486/indhealth.2020-0252

Figure Lengend Snippet: Protein expression in the liver of mice exposed to 2EH for 3 months (3 M) or 6 months (6 M). Representative bands of catalase, BP, VLCAD, MTTP, and GAPDH after western blot analysis in mice exposed to 2EH for 3 M and 6 M. 2EH, 2-Ethyl-1-hexanol; MTTP, microsomal triglyceride transfer protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; VLCAD, very long-chain acyl-CoA dehydrogenase; BP, peroxisomal bifunctional protein.

Article Snippet: The primary antibodies used for western blot analyses were as follows: microsomal triglyceride transfer protein (MTTP) (Aviva System Biology Corporation, San Diego, California, USA), catalase (Abcam plc., Cambridge, Cambridgeshire, UK), peroxisomal bifunctional protein (BP) ) , very-long-chain acyl-CoA dehydrogenase (VLCAD) ) , and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cell Signaling Technology, Inc., Danvers, Massachusetts, USA).

Techniques: Expressing, Western Blot